测氨基酸序列的仪器(技术升级丨高分辨质谱助力大分子蛋白药物结构确证研究)

寰氨澶у垎瀛愯嵂鐗╁垎鏋愭湇鍔℃嫢鏈?/span>绗﹀悎鍥介檯璐ㄩ噺鏍囧噯鐨勭敓鐗╄嵂鍒嗘瀽鏈嶅姟骞冲彴锛屽彲浠ヤ负骞垮ぇ鍖昏嵂瀹㈡埛鎻愪緵澶у垎瀛愯嵂鐗╃粨鏋勮〃寰併€佹潅璐ㄥ垎鏋愶紙宸ヨ壓鏉傝川娈嬬暀銆佽泲鐧芥畫鐣欍€佽泲鐧芥潅璐ㄩ壌瀹氾級銆佸垎鏋愭柟娉曞紑鍙戝拰楠岃瘉銆佺ǔ瀹氭€х爺绌躲€佽緟鏂?寰敓鐗╂娴嬨€佺浉瀹规€?瀵嗗皝鎬х爺绌剁瓑涓€浣撳寲鐨勭敓鐗╁埗鍝佺爺绌舵湇鍔°€?/span>

鈻?寰氨鐢熺墿澶у垎瀛愬疄楠屽

澶у垎瀛愯嵂鐗╁垎鏋愭湇鍔?/span>

浠櫒浠嬬粛

ACQUITYTM UPLCTM H-Class PLUS SystemXevoTM G2-XS QTof Mass SpecctrometerWaters UNIFI庐

ACQUITYTM UPLCTM H-Class PLUS绯荤粺鍏锋湁鍥涘厓婧跺墏娣峰悎鐨勭伒娲绘€у拰绠€鏄撴€э紝骞跺甫鏈変竴涓祦閫氶拡寮忚繘鏍峰櫒锛岄厤鏈夌传澶栵紙TUV锛変笌鑽у厜锛團LR锛夋娴嬪櫒锛屽疄鐜颁簡UPLC楂樺垎绂诲害銆侀珮鐏垫晱搴﹀拰楂橀€氶噺锛屽悓鏃跺叿鏈堿CQUITY UPLC绯荤粺鐨勮€愮敤鎬т笌鍙潬鎬с€?/span>

XevoTM G2-XS QTof 璐ㄨ氨鍏锋湁MS銆丮S/MS銆丮SE銆丮RM銆丗astDDA绛夊绉嶉噰闆嗘ā寮忥紝鍚屾椂鍏煎叿楂樺垎杈ㄧ巼銆侀珮鐏垫晱搴︿笌楂樼簿纭川閲忔暟銆?/span>

Waters UNIFI庐 鏄珮鍒嗚鲸璐ㄨ氨鏁版嵁閲囬泦銆佸鐞嗐€佹祻瑙堛€佹姤鍛婄敓鎴愯蒋浠讹紝鍙疄鐜版暟鎹殑鍙拷婧€с€?/span>

锛堜俊鎭潵鑷猈aters瀹樼綉锛?/span>

鍘熺悊涓庡簲鐢?/span>

鍘熺悊

楂樺垎杈ㄨ川璋?/span>鏄互瓒呴珮鏁堟恫鐩歌壊璋变綔涓鸿繘鏍蜂笌鍒嗙绯荤粺锛?/span>璐ㄨ氨浣滀负妫€娴嬬郴缁燂紝澶嶆潅鐨勭敓鐗╂牱鍝佸湪娑茬浉鑹茶氨涓庤川璋遍儴鍒嗙粡杩囧垎绂诲拰绂诲瓙鍖栵紝缁忕敱璐ㄨ氨妫€娴嬪櫒寰楀埌璋卞浘锛屼粠鑰屽疄鐜扮墿璐ㄧ殑瀹氭€т笌瀹氶噺鍒嗘瀽銆傚湪CID锛堢鎾炶瀵艰В绂伙級妯″紡涓嬶紝鐗╄川鍒嗗瓙鍙繘涓€姝ヨ纰庤鎴愬搴旂殑纰庣墖绂诲瓙锛岄€氳繃妫€绱㈢鐗囦俊鎭笌鏁版嵁搴撶殑鍖归厤锛屽彲浠ュ疄鐜扮墿璐ㄥ垎瀛愮殑涓€绾у簭鍒楃殑瀹氭€у垎鏋?/span>銆?/span>

搴旂敤

璇ヤ华鍣ㄤ富瑕佸簲鐢ㄤ簬铔嬬櫧璐ㄥ垎瀛愮殑妫€娴?/span>锛屽澶氳偨銆佸崟鍏嬮殕鎶椾綋銆佸弻鐗瑰紓鎬ф姉浣撱€佽瀺鍚堣泲鐧姐€佹姉浣撳伓鑱旇嵂鐗╃瓑銆傚彲瀵瑰叾瀹屾暣鍒嗗瓙閲?/span>銆?/span>杩樺師鍒嗗瓙閲?/span>銆?/span>浜氬熀鍒嗗瓙閲?/span>銆?/span>浜岀～閿厤瀵规垨閿欓厤銆?/span>姘ㄥ熀閰稿簭鍒?/span>锛?/span>娓哥宸熀浣嶇偣涓庢瘮渚?/span>銆?/span>N-绯栧熀鍖栦綅鐐逛笌绫诲瀷銆?/span>缈昏瘧鍚庝慨楗颁綅鐐逛笌姣斾緥锛堣劚閰拌兒銆佹哀鍖栥€丯/C绔慨楗扮瓑锛夌瓑杩涜瀹氭€т笌鐩稿瀹氶噺鍒嗘瀽銆?/span>

妗堜緥浠嬬粛

1 绮剧‘鍒嗗瓙閲?/span>

鈻插浘1 绮剧‘鍒嗗瓙閲忥細浠庝笂鑷充笅鍒嗗埆涓篢IC銆丮ass spectra鍙奃econvoluted mass鍥捐氨

鈻插浘2 澶氳偨鍏ㄥ簭鍒桵S/MS鍒嗘瀽锛氱敱涓婅嚦涓嬪垎鍒负TIC銆丆omponent plot銆丮ass spectra鍙奆ragmentation viewer 鍥捐氨

2 搴忓垪瑕嗙洊鐜?/span>

鈻插浘3 浠ｈ〃鎬ф皑鍩洪吀搴忓垪瑕嗙洊鐜囧浘璋?/span>

姘ㄥ熀閰稿簭鍒楄鐩栫巼纭锛氶鍏堟牴鎹悊璁烘皑鍩洪吀搴忓垪閫夋嫨涓€绉嶆垨鑰呭绉嶉叾杩涜閰跺垏涓婃満锛岀劧鍚庨€氳繃璐ㄨ氨鐨勪竴绾S涓庝簩绾S/MS鏁版嵁杩涜搴忓垪瑕嗙洊鐜囧垎鏋愶紝浣垮叾缁煎悎搴忓垪瑕嗙洊鐜囪揪鍒?5锛呬互涓娿€?/span>

鈻茶〃1 甯哥敤铔嬬櫧閰跺強鐗瑰紓鎬ч叾鍒囦綅鐐?/span>

3 璐ㄩ噺鑲藉浘

璐ㄩ噺鑲藉浘鍒嗘瀽鍙湁鏁堥壌瀹氳泲鐧借川鐨勪竴绾х粨鏋勪俊鎭紝鍖呮嫭閴村畾CDR鍖鸿偨娈典綅缃€佹槸鍚﹀彂鐢熷寲瀛︿慨楗版垨鑰呮皑鍩洪吀绐佸彉绛夛紝鑳芥湁鏁堣川鎺х敓鐗╁埗鍝佺殑绋冲畾鎬у拰瀹屾暣鎬с€?/span>

鈻插浘4 渚涜瘯鍝乀rypsin閰惰В浜х墿TIC鍥捐氨

鈻茶〃2 渚涜瘯鍝佺粡Trypsin閰舵秷鍖栧悗鐨勮偨娈靛嘲鐨勮川璋遍壌瀹氫俊鎭?/span>

4 浜岀～閿厤瀵瑰垎鏋?/span>

浜岀～閿槸涓€绉嶉噸瑕佺殑铔嬬櫧璐ㄧ炕璇戝悗淇グ锛屽缁存寔铔嬬櫧璐ㄧ┖闂寸粨鏋勮捣鐫€閲嶈鐨勪綔鐢紝涔熷鍏剁敓鐗╂椿鎬у奖鍝嶉噸澶э紝鍥犳纭瘉铔嬬櫧鍒嗗瓙姝ｇ‘鐨勪簩纭敭閰嶅鏂瑰紡鏄壒鎬ч壌瀹氫腑閲嶈鐨勭爺绌跺唴瀹广€?/span>

鈻插浘5 渚涜瘯鍝佺粡閰跺垏鍚庯紙杩樺師鍜岄潪杩樺師锛塗IC瀵规瘮鍥?/span>

5 N-绯栬氨鍒嗘瀽

绯栬泲鐧界殑绯栭摼瀵圭淮鎸佽泲鐧界┖闂寸粨鏋勭殑绋冲畾鎬у拰鐢熺墿娲绘€ц捣鐫€閲嶈鐨勪綔鐢紝鍚屾椂涔熻兘褰卞搷璁稿铔嬬櫧鑽墿鐨勫畨鍏ㄦ€с€?/span>

鈻插浘6 鐢变笂鑷充笅涓篘-绯栧瀷鐨凾IC鍥惧拰FLR鍥?/span>

鎶€鏈崌绾т辅楂樺垎杈ㄨ川璋卞姪鍔涘ぇ鍒嗗瓙铔嬬櫧鑽墿缁撴瀯纭瘉鐮旂┒,鏂拌川璋卞垎鏋愭妧鏈?/strong>