贵州氨基酸推荐(贵州大学方中明研究组发现氨基酸转运蛋白可调控水稻分蘖和产量)

璐ｇ紪 | 鐜嬩竴

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杩戞棩锛岃吹宸炲ぇ瀛﹀啘瀛﹂櫌鏂逛腑鏄?/strong>鏁欐巿鐮旂┒缁勫湪Rice鍙戣〃浜嗛涓?strong>The amino acid transporterOsAAP4 contributes to rice tillering and grain yield by regulating neutral amino acid allocation through two splicing variants鐨勭爺绌惰鏂囥€傝鐮旂┒鍙戠幇浜嗘按绋绘皑鍩洪吀杞繍瀹舵棌鎴愬憳OsAAP4鐨勪袱绉嶅壀鎺ヤ綋涓昏閫氳繃璋冩帶涓嶅悓娴撳害鐨勪腑鎬ф皑鍩洪吀鍒嗛厤锛屽苟閫氳繃姘唬璋㈠拰婵€绱犻€斿緞姝ｈ皟鎺ф按绋诲垎铇栧拰浜ч噺銆?/p>

璇ョ爺绌跺彂鐜帮紝姘寸ɑ姘ㄥ熀閰歌浆杩愬熀鍥?em>OsAAP4鐨勫惎鍔ㄥ瓙搴忓垪鍦ㄧ奔绋诲拰绮崇ɑ涔嬮棿瀛樺湪宸紓锛屽湪姘寸ɑ鍒嗚槚鑺藉拰鍩洪儴涓熀鍥犵殑琛ㄨ揪姘村钩涓庣奔绋汇€佺渤绋荤殑鍒嗚槚鍜屼骇閲忓憟姝ｇ浉鍏炽€傚惎鍔ㄥ瓙-GUS鏌撹壊琛ㄦ槑锛?em>OsAAP4涓昏鍦ㄤ晶鏍广€佸垎铇栬娊銆佸彾鐗囥€佸彾闉樸€佽寧鍜岀涓〃杈俱€傜煶铚″垏鐗囧疄楠屾樉绀猴紝GUS娲绘€ч泦涓湪鏍圭毊灞傝杽澹佺粏鑳烇紝鍙婂彾闉樸€佸彾鐗囥€佽寧鍜屽辜绌楃淮绠＄粍缁囩殑鏈ㄨ川閮ㄥ拰闊х毊閮ㄤ腑銆備簹缁嗚優瀹氫綅瀹為獙琛ㄦ槑锛孫sAAP4涓ょ鍓帴浣撳潎瀹氫綅鍦ㄧ粏鑳炶啘鍙婄粏鑳炴牳涓€侽sAAP4鐨勪袱涓笉鍚屽壀鎺ヤ綋鍦ㄧ渤绋籞H11涓繃琛ㄨ揪鏄捐憲鎻愰珮浜嗘按绋诲垎铇栧拰浜ч噺锛孯NAi鍜岀獊鍙樹綋妞嶆牚琛ㄧ幇鍑虹浉鍙嶇殑瓒嬪娍銆?/p>

Phenotypic analysis of OsAAP4 transgenic plants in the Japonica ZH11 background grown in Wuhan paddy fields.

鍒╃敤楂樻晥娑茬浉鑹茶氨瀹為獙瀵逛笉鍚岄樁娈垫鏍殑姘ㄥ熀閰稿惈閲忔祴瀹氳〃鏄庯紝涓€ф皑鍩洪吀缂皑閰搞€佽劘姘ㄩ吀銆佽嫃姘ㄩ吀鍜屼寒姘ㄩ吀鍙戠敓浜嗘槑鏄炬敼鍙樸€傝繘涓€姝ヨ〃鏄庯紝0.5 mM鐨勫婧愮棘姘ㄩ吀鎴栬劘姘ㄩ吀鏄捐憲淇冭繘杩囪〃杈炬鏍?em>OsAAP4a鐨勫垎铇栬娊浼搁暱锛岃€?.0mM鐨勫婧愮棘姘ㄩ吀鎴栬劘姘ㄩ吀鍒欐樉钁椾績杩涜繃琛ㄨ揪妞嶆牚OsAAP4b鐨勫垎铇栬娊浼搁暱銆傚彟澶栵紝鍘熺敓璐ㄤ綋-鑽у厜姘ㄥ熀閰稿惛鏀剁殑缁撴灉琛ㄦ槑锛屼笉鍚屾祿搴︾殑缂皑閰稿拰鑴皑閰稿湪杩欎簺杩囪〃杈炬鏍腑鐗瑰紓鎬у湴杩愯緭鍜岀Н绱€傝浆褰曠粍鍒嗘瀽杩涗竴姝ヨ〃鏄庯紝OsAAP4琛ㄨ揪閲忔敼鍙樺彲浠ラ€氳繃褰卞搷姘礌鐨勮繍杈撳拰浠ｈ阿锛屼互鍙婄敓闀跨礌銆佺粏鑳炲垎瑁傜礌绛夋縺绱犻€斿緞璋冩帶姘寸ɑ鍒嗚槚銆?/p>

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鎹倝锛屾柟涓槑鏁欐巿鍥㈤槦瀵规按绋绘皑鍩洪吀杞繍铔嬬櫧鐨勮皟鎺ц繘琛屼簡杈冧负娣卞叆鐨勭爺绌讹紝甯﹂鍥㈤槦鎴愬憳鍚村崥鏂囥€佹豹鏉般€佸悤鍑瓑鏇惧厛鍚庤В鏋愪簡纰辨€ф皑鍩洪吀杞繍铔嬬櫧OsAAP3璐熻皟鎺ф按绋诲垎铇栧拰姘埄鐢ㄦ晥鐜?span style=”color: #888888; –tt-darkmode-color: #888888;”>锛圠u et al., Plant Biotechnology Journal, 2018锛?/span>銆佺⒈鎬у強涓€ф皑鍩洪吀杞繍铔嬬櫧OsAAP5璐熻皟鎺ф按绋诲垎铇栧拰鍗曟牚浜ч噺 (Wang et al., Plant Physiology, 2019)銆佷腑鎬ф皑鍩洪吀杞繍铔嬬櫧OsAAP1姝ｈ皟鎺ф按绋诲垎铇?span style=”color: #888888; –tt-darkmode-color: #888888;”>锛圝i et al., Journal of Experimental Botany, 2020锛?/span>锛屽鏈夋満姘惀鍏讳腑姘ㄥ熀閰稿強鍏舵皑鍩洪吀杞繍铔嬬櫧宸紓鎬ц皟鎺ф按绋诲啘鑹烘€х姸鍙婃爱鍒╃敤鏁堢巼鍋氬嚭浜嗚础鐚€?/p>

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